| 10-07-2018 |
Characterization of computational and experimental activity of the beta-carotene hydroxylase from Pantoea ananatis
Beta-carotene hydroxylase is one of enzymes which constitutes the rate-limiting step in the production of astaxanthin, a compound of high commercial value for the industry. Recently, the laboratory of Dr. Eduardo Agosin obtained a yeast strain that express beta-carotene hydroxylase (gene crtZ) of the bacterium P. ananatis, which is a membrane protein that converts beta-carotene into zeaxanthin. However, neither the structure of this protein and the residues responsible for this activity have been characterized previously. This collaborative research between the Drs. César A. Ramírez-Sarmiento, and Eduardo Agosin seeks to generate a model of the three-dimensional structure of this protein and the determination of the residues responsible for its activity by molecular docking, followed by the validation of the residues identified computationally by site-directed mutagenesis and evaluation of production of zeaxanthin in yeast strains expressing this protein.
Prerequisites:
None.
Evaluation method: Nota 1-7, with 0/1 available vacants |
Mentor(s): Open in the plataform |